Immunology, Physiology and Molecular that causes of T.gondii infection. Evaluate the molecular diagnosis of T.gondii and cytokines by using PCR

      Аннотация: This study aims to find out the immune response (antibodies and cytokines) for women with disease T.gondii,as well as the study of molecular. Were collected (200) samples of blood and (30) samples of umbilical cord of women and pregnant women with disease T.gondiiand revisions to hospitals in Najaf and laboratories for the purposes of abortion or treatment of disease, while the number of women samples control amounted to 30 samples and pregnant women and control were divided into three age groups, namely, (15-25), (26-36) and (37-47) respectively.The study involved collecting samples and tested the first test by latex and then examined by the technique of (ELISA) for the detection of antibodies (IgG and IGM). The study reported that technique is more important than the ELISA test for latex which was (P = 0.007). The study showed that there is a relationship between genes Toxo-B1, ITS-1, SAG-1 and SAG-2 by examining the DNA taken from the blood and placenta of women with T.gondii. And recorded the highest number of positive samples (52) in the sample ITS-1 from the rest of the other genes.

Ключевые слова: immunology, PCR, Cytokines, T.gondii