Camels Nasal Myiasis. Immunogenic modulators and genetic investigation

      Аннотация: Cephalopina titillator larvae (Diptera: Oestridae) are nasal myiasis-producing agent of camels. Diagnosis of this parasite is very difficult in living animals. ELISA showed that the higher apparent prevalence values 89.66% and 79.31% were recorded with L2CE and SGc antigens, respectively. Both induced the highest specificity and positive predictive value reported 100% for each. The SDS-PAGE and WB colorimetric reaction of SGc antigen had the most intensely stained theme at 45-48 KDa complex polypeptide array band.The amplified COI gene and mitochondrial 16S rRNA gene were differed according to the primer combination. The constructed Phylogenetic trees of both amplicon sequence analysis suggested that the subfamily Oestrinae is not monophyletic. Therefore, the collection of additional molecular data is obligatory before we can establish a reliable phylogenetic hypothesis or suggest new taxonomic classi?cation of the genus Cephalopina

Ключевые слова: DNA, ELISA, COI, camel, nasal myiasis, Cephalopina titillator, SDS, WB, 16S rRNA