Engineering a Plant Activation Tagging method using transposons.

      Аннотация: Strategies that aid the discovery of new gene functions in plants are important tools that can help to improve traits to cope with the challenges that agriculture and plant-based industries face nowadays. They are also useful from a scientific point of view, contributing to the knowledge about the genetic regulation of plant processes in general. In this work, a transposon-based strategy to obtain gain-of-function mutants in the model plant Arabidopsis thaliana is presented. This strategy allows the discovery of new gene functions that can be difficult to find with other, such as loss-of-function, strategies.

Ключевые слова: gene regulation, Activation Tagging, transposons, mutant screening, gain-of-function mutation, Biotechnology, plants, genetic regulation