Research Techniques in Molecular Biology, Genetics and Biotechnology. A Laboratory Manual for Cytogeneticists

      Аннотация: The present book comprises Research Techniques in Cell & DNA Biology, Genetics and Biotechnology covering the Study of Mitosis, Meiosis, Ascites Cell Transplantation, Tumor Cells, using in vivo and in vitro culture techniques. It also covers procedures to investigate Micro-, Macro- and Sex- chromosomes, their Morphometric Analysis and Cytochemical Different-iations. Methodologies pertaining to Extraction, Separation and Replication of DNA employing Gel Electrophoresis, PCR, RT-PCR, have been dealt in detail. In each experiment, chemical and glassware specifications have been clearly earmarked. Each experimental protocol has been provided with foot-notes to facilitate the reader to comprehend the succession of changes that take place with addition of a reagent. Preparation of Stains & Reagents will ease the scholar to learn and prepare them on his own. A comprehensive list of books, appended at the end, will permit research enthusiasts to make further probe into the area of their interest.

Ключевые слова: electrophoresis, Chromosomes, Avian Cytology, Banding Analysis, Cell Biopsy, Cytochemical Differentiation, Cytobiometry, DNA Extraction & Separation, Oncology.