Genetic Diversity and Genetic Relationships in Garlic Germplasm. Genetic diversity using Morpho-agronomic traits and Molecular markers (RAPD, ISSR & SSR)

      Аннотация: The present investigation was conducted to assess the genetic diversity among 131 garlic genotypes using twelve morpho-agronomic traits and molecular markers at SVPUAT, Meerut, during rabi season of 2014. At the morphological level, ANOVA showed significant variation (p < 0.05) for all traits indicating greater variability in germplasm. Mahalanobis D 2 analysis revealed a considerable amount of diversity in the germplasm. Traits like clove weight and bulb weight per plant were the major contributors to genetic divergence. At the molecular level, 19 RAPD and 4 ISSR marker generated 74 and 12 polymorphic bands respectively and SSR produced 42 alleles were detected among 131 garlic genotypes with an average of 3.9 (RAPD), 3 (ISSR) and 2 (SSR) bands. Cluster analysis based on UPGMA, three separate dendrograms, and one combined similarity tree, prepared will help the genetic relation studies among germplasm. The genotypes viz., K1, GHC-1, PG-20, W6-35698, M352, AVTG-2, Chachena mota, Kantgola, CFG-3, AVTG-4 were more diverse with the good horticultural performance thus, these genotypes could be a good parental choice for further garlic breeding programs.

Ключевые слова: Allium sativum, garlic, Genetic Diversity, Genetic ?diversity, Molecular markers, Morpho-Agronomic Traits