Identification and Characterization of a Novel LPA2 Receptor Agonist. Antiapoptotic and Radiomitigative Properties of GRI977143 a Novel Nonlipid LPA2 Receptor Agonist

      Аннотация: The endogenous lipid mediator lysophosphatidic acid (LPA) regulates fundamental cellular responses, ranging from survival through proliferation to motility and migration. Acting through one of its cell membrane receptors, the LPA2 receptor, LPA protects against chemotherapy- and ?-irradiation-induced apoptosis. Virtual screening led to the identification of GRI977143 (GRI), a novel nonlipid LPA2 receptor agonist. GRI reduced activation of caspases 3, 7, 8, and 9, and inhibited Bax translocation, poly (ADP-ribose) polymerase 1 cleavage, and DNA fragmentation in the serum withdrawal, Adriamycin, TNF-?, and ?-irradiation apoptosis models. GRI acted as a radiomitigator and rescued the lives of mice when administered 24 h after lethal radiation exposure. GRI also inhibited bystander apoptosis elicited by proapoptotic mediators produced by irradiated cells. Prosurvival and radiomitigative effect of GRI may in part be attributed to the LPA2 receptor-mediated activation of the extracellular signal regulated kinases 1 and 2. GRI represents an excellent candidate for further development of radiomitigative drugs, and agents that prevent apoptosis in degenerative and inflammatory diseases.

Ключевые слова: apoptosis, lysophosphatidic acid, ?-irradiation, Computational Modeling, LPA2 receptor, ligand-based similarity search, radiomitigation, bystander apoptosis, hematopoietic acute radiation syndrome, extracellular signal regulated kinases 1 and 2