Diuretic and Antiurolithiatic activities of Saccharum officinarum. Evaluation of Invivo Diuretic, and Antiurolithiatic Activities of Ethanolic Leaf Extract of Saccharum officinarum

      Аннотация: The present work was investigated to evaluate the diuretic and antiurolithiatic activities on leaf extract of Saccharum officinarum. The leaf extract was administered to experimental rats orally at a dose of 200mg/ kg and 400mg/kg. Furosemide (5mg/kg) was used as standard for diuretic activity. The parameters measured for diuretic activity was total urine volume; urine electrolyte concentrations such as sodium, potassium, chloride and bicarbonates. In in-vitro antiurolithiatic activity, Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine. The effect of extract (100, 200 and 500?g/ml) was studied by time course measurement of absorbance at 620 nm for ten minutes by means of a spectrophotometer. The extract showed inhibition at 120 sec (40.31, 60.46 and 63.56 %), and maximum inhibition of the crystallization of calcium oxalate at 600 seconds (55.24, 69.93 and 72.72%). In in-vivo antiurolithiatic activity, urolithiasis was induced in male rats by administering ethylene glycol (0.75% v/v) in drinking water for 28days and the parameters such as oxalate, calcium and phosphate were estimated.

Ключевые слова: diuretic, IN-VITRO, in-vivo, leaf extract, Saccharum officinarum, antiurolithiatic, activity.