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Evaluation of performance of serological tests.
В наличии
Местонахождение: Алматы | Состояние экземпляра: новый |
Бумажная
версия
версия
Автор: Kiriinya Nicholas Ruuri and Ann Muigai
ISBN: 9786138234043
Год издания: 2018
Формат книги: 60×90/16 (145×215 мм)
Количество страниц: 76
Издательство: LAP LAMBERT Academic Publishing
Цена: 21841 тг
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Аннотация: This book describes Evaluation of performance of serological tests for the detection of Foot and Mouth Disease virus antibodies in Kilifi County, Kenya. Objective: To determine the sensitivity of assays Anigen © FMD NSP Ab ELISA –Korea, NSP Priocheck® kits with Liquid Phase Blocking Enzyme Linked Immunosorbent Assay compared with Virus neutralization test ( the gold standard) and Identification of prevalent serotypes of foot and mouth disease virus in Kilifi County coast of Kenya 2014. Design: cross-sectional study design. Setting of the study: Kilifi County. Subjects: 421 cattle serum. Results: The results showed that both Anigen © FMD NSP Ab ELISA (Korea), NSP Priocheck® Netherlands assays had low sensitivity but high specificity. Liquid Phase Blocking ELI SA had the highest sensitivity 100%. LPBE had a Positive Predictive Value (PPV) =100%, Anigen © FMD NSP Ab ELISA–Korea 94.44% and NSP Priocheck® Netherlands had the lowest 44.74% compared with the VNT 100%. The Cohen's kappa coefficient showed a perfect agreement level of 90%, 93%, 100 and 100% respectively. Conclusion: This study showed no significant difference in sensitivity and both structural and non-structural tests.
Ключевые слова: Sensitivity, Specificity, Liquid Phase Blocking Enzyme Linked Immunosorbent Assay, virus neutralization Test, anigen, NSP priocheck