INSTRUMENTAL METHOD OF ANALYSIS. LAB MANUAL

      Аннотация: The aim is to determine λ max of KMnO4 solution.Instrument: colorimeter Apparatus: 100ml volumetric flask, test tubes, beakers Chemicals: KMnO4 solution, distilled water Principle: Spectroscopy is the tool for study of atomic and molecular structure. It deals with interaction of electronic radiation with matter involving the measurement and interpretation of the extension of absorption or emission of electromagnetic radiation by molecule. λmax is defined as wavelength at which maximum absorption of radiation takes place. The extent to which a sample absorbs light depends strongly upon the wavelength of light and type chromophore present in the analyte. Chromophore is a functional group or a part of the molecule responsible for the absorption of electromagnetic radiation at a specific frequency. λmax is characteristic of a compound and provides information of the electronic transitions occurs in the analyte. In order to obtain the highest sensitivity and to minimize deviations from Beer's Law, analytical measurements are made using light with a wavelength of λmax.NOTE: λmax is independent from the concentration of the analyte.

Ключевые слова: absorptionn, UV Spectrophotometry, HPLC, interpretation