Electron Microscopy Mastery Guide. Overcoming Sample Fixative Pitfalls: Personal Experience

      Аннотация: The core objective of this book is to provide practical guidance on the complex steps involved in accurate specimen fixation for electron microscopic examination. It thoroughly explains primary and secondary fixatives, along with their critical additives, and offers comprehensive instructions for the block processing of fixed cells. Readers will find valuable ideas, practical advice, step-by-step guidelines, and best practices that empower them to confidently tackle common pitfalls and complications in the sample fixation process. Bridging the gap between theory and practice, this resource is equally beneficial for researchers and technicians. While it contributes significantly to the field of electron microscopy research, the true impact of this book lies in its ability to enhance the daily practices of professionals. By improving sample fixation and processing. This book is an indispensable tool for anyone looking to master the art of electron microscopy sample preparation.

Ключевые слова: Electron Microscopy, Cell Fixation, Electron Microscopy Fixatives, EM Pitfalls and Solution, Electron Microscopy Technique, Agarose Cell Block Technique, Osmolarity, Buffer, Glutaraldehyde, Epoxy resin, Osmic acid, Paraformaldehyde, cacodylate, Overcoming the pitfalls in electron microscopy sample fixatives, Bridge the gap between theory and practice by step by step guidance to improve EM sample fixation, Specimen preparation for electron microscopic examination.